《植物生理学报》 2015, 51(10): 1687-1696
通信作者：杨建昌；E-mail: jcyang@yzu.edu.cn；Tel: 0514-8797931
摘 要：
以籼稻‘双桂1号’ (热敏感品种)和‘黄华占’ (耐热品种)为材料, 研究减数分裂期高温胁迫对水稻幼穗内源激素、多胺积累和蛋白质表达的影响。结果表明: 在高温下, ‘黄华占’的受精率和饱粒率显著高于‘双桂1号’。高温引起了两品种幼穗中吲哚乙酸(IAA)及玉米素(Z)和玉米素核苷(ZR)减少, 脱落酸(ABA)含量增加, 且‘黄华占’的变化小于‘双桂1号’; 高温使两品种精胺(Spm)含量增加, ‘双桂1号’的腐胺(Put)和亚精胺(Spd)含量减少, 但‘黄华占’ Put和Spd含量增加。两品种蛋白质表达对高温响应有差异, 这些差异蛋白质主要涉及氧化还原调控、糖酵解、氨基酸代谢、光系统和胁迫响应。‘黄华占’的氧化还原调控蛋白在高温下表达量较高。表明耐热品种在高温下受精结实较好与其较高的IAA、Z+ZR和游离多胺含量, 较低的ABA含量及高表达的氧化还原调控相关的蛋白有密切关系。关键词：水稻; 高温; 激素; 多胺; 蛋白质
收稿：2015-07-31 修定：2015-10-07
资助：国家自然科学基金(31271641和31471438)、江苏高校优势学科建设工程专项(PAPD-2014)、江苏省科技支撑计划项目(BE2014411)和江苏省高校自然科学研究基金(13KJB210005)。
Corresponding author: YANG Jian-Chang; E-mail: jcyang@yzu.edu.cn; Tel: 0514-8797931
Abstract:
Using two rice (Oryza sativa L. ssp. indica) varieties, ‘Shuanggui No. 1’ (heat-sensitive) and ‘Huanghuazhan’ (heat-tolerant), as materials, the effect of high temperature during meiosis on hormones, polyamines accumulation and protein expression in young panicles of rice was investigated in this study. Under high temperature treatment, the fertilized-spikelet and filled-grain percentages were significantly higher in heat-tolerant variety than those in heat-sensitive one. Abscisic acid (ABA) content was increased by high temperature treatment, whereas indole acetic acid (IAA) and zeatin (Z) + zeatin riboside (ZR) contents were decreased. These hormone level changes were less obvious in ‘Huanghuazhan’ than those in ‘Shuanggui No. 1’. Spermine (Spm) levels in both varieties were up-regulated under high temperature. The levels of putrescine (Put) and spermidine (Spd) were decreased in ‘Shuanggui No. 1’ under high temperature, but they were increased in ‘Huanghuazhan’. Differences of protein expression in response to high temperature between the two varieties have been observed including proteins involved in redox regulation, glycolysis, amino acid metabolisms, photosynthesis and stress responses. Proteins related to redox regulation were preferentially expressed in ‘Huanghuazhan’ under high temperature treatment. In summary, our results indicate that, under high temperature, higher fertilized-spikelet and filled-grain percentages in heat-tolerant variety are closely associated with a higher concentration of IAA, Z+ZR and polyamines, and a lower concentration of ABA and higher expression of proteins involved in redox regulation in young panicles of rice during meiosis.Key words: rice (Oryza sativa); high temperature; hormones; polyamines; protein
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